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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been noticed in lots of tumors, which ends up in chromosome instability, and thus might add to tumorigenesis. This protein was discovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant job in selling mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two added splice variants have been documented but in depth nucleotide sequence details isn't nonetheless available.

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Blocking peptides are peptides that bind particularly on the target antibody and block antibody binding. These peptide commonly has the epitope acknowledged with the antibody.

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This information outlines further more doable optimization of cellular and immunoassay parameters to be certain the absolute best results are...

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the absolute best final results are...

Antibodies sure to the blocking peptide not bind to your epitope around the focus on protein. By evaluating the staining from the blocked antibody compared to the antibody by yourself, you can see which staining is distinct.

Concentrate on Information Cyclin E belongs to the highly conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of check here every mitotic function. Cyclin E kinds a fancy with and features like a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates within the G1-S section boundary and is particularly degraded as cells development by way of S period.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...

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